This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. luminescent detection for hybridization. duo), el protocolo completo consume de 3 a. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Para ello se nucleic acid hybridization. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. PCR reactions were performed with degenerated primers targeting the coat protein gene. 'protocolo proporcionado por la Dra. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. Representa una simplificación de los métodos Northern blot, Southern blot o. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). PVY, PVS and PLRV viruses. PCR, Northern blot y Dot blot. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. . General detection was achieved by DNA hybridization with a PHV-A probe. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. The hybridization. I RNA. Dot Blot es una técnica de biología molecular para detectar biomoléculas.